Publications
PUBLICATIONS 1991
Van Vooren JP, Drowart A, De Cock M, Van Onckelen A, D’Hoop MH, Yernault JC, Valcke C, Huygen K. Humoral immune response of tuberculous patients against the three components of the Mycobacterium bovis BCG 85 complex separated by isoelectric focusing. J Clin Microbiol. 29,2348-50,1991
PUBLICATIONS 1992
Drowart A, De Bruyn J, Huygen K, Damiani G, Godfrey HP, Stélandre M, Yernault JC, Van Vooren JP. Isoelectrophoretic characterization of protein antigens present in mycobacterial culture filtrates and recognized by monoclonal antibodies directed against the Mycobacterium bovis BCG antigen 85 complex. Scand J Immunol. 36,697-702,1992.
Stélandre, M. Bosseloir Y, De Bruyn J, Maes P, Content J. Cloning and sequence analysis of the gene encoding an NADP-dependent alcohol dehydrogenase in Mycobacterium bovis BCG. Gene, 121,79-86,1992.
PUBLICATIONS 1993
Fontaine V, Savino R, Arcone R, de Wit L, Brakenhoff JP, Content J, Ciliberto G. Involvement of the Arg179 in the active site of human IL-6. Eur J Biochem. 211,749-55,1993.
PUBLICATIONS 1994
Braibant M, De Wit L, Peirs P,Kalai M, Ooms J, Drowart A, Huygen K, Content J. Structure of the Mycobacterium tuberculosis antigen 88, a protein related to the Escherichia coli PstA periplasmic phosphate permease subunit. Infect Immun. 62,849-54,1994.
Brakenhoff JP, de Hon FD, Fontaine V, ten Boekel E, Schooltink H, Rose-John S, Heinrich PC, Content J, Aarden LA. Development of a human interleukin-6 receptor antagonist. J Biol Chem. 269, 86-93,1994.
Fontaine V, Ooms J, Content J. Mutagenesis of the human interleukin-6 fourth predicted alpha-helix : involvement of the Arg168 in the binding site. Eur J Immunol. 24,1041-5,1994.
Huygen K, Lozes E, Gilles B, Drowart A, Palfliet K, Jurion F, Roland I, Art M, Dufaux M, Nyabenda J, et al. Mapping of TH1 helper T-cell epitopes on major secreted mycobacterial antigen 85A in mice infected with live Mycobacterium bovis BCG. Infect Immun. 62,363-70,1994.
Mahieu M, Deschuyteneer R, Forget D, Vandenbussche P, Content J. Construction of a ribozyme directed against human interleukin-6 mRNA : evaluation of its catalytic activity in vitro and in vivo. Blood 84,3758-65,1994.
Snyers L, Content J. Induction of metallothionein and stomatin by interleukin-6 and glucocorticoids in a human amniotic cell line. Eur J Biochem. 223,411-8,1994.
PUBLICATIONS 1995
Hendrix C, Mahieu M, Anné J, Van Calenbergh S, Van Aerschot A, Content J, Herdewijn P. Catalytic activity and stability of hammerhead ribozymes containing 2’-acetamido-2’-deoxyribonucleosides. Biochem Biophys Res Commun. 210,67-73,1995
Mahieu M, Hendrix C, Ooms J, Herdewijn P, Content J. In vitro NCp7 enhancement of ribozyme-mediated cleavage of full-length human IL-6 mRNA. Biochem Biophys Res Commun. 214,36-43,1995.
Snyers L, Renauld JC, Szpirer C, Content J. Cloning and chromosomal localization of a pseudogene corresponding to a mRNA for a soluble IL-6 receptor. DNA Seq. 5,311-4,1995.
PUBLICATIONS 1996
Braibant M, Lefèvre P, de Wit L, Ooms J, Peirs P, Huygen K, Wattiez R, Content J. Identification of a second Mycobacterium tuberculosis gene cluster encoding proteins of an ABC phosphate transporter. FEBS Lett. 394,206-12,1996.
Braibant M, Lefèvre P, de Wit L, Peirs P, Ooms J, Huygen K, Andersen AB, Content J. A Mycobacterium tuberculosis gene cluster encoding proteins of a phosphate transporter homologous to the Escherichia coli Pst system. Gene 176,171-6,1996.
Huygen K, Content J, Denis O, Montgomery DL, Yawman AM, Deck RR, DeWitt CM, Orme IM, Baldwin S, D’Souza C, Drowart A, Lozes E, Vandenbussche P, Van Vooren JP, Liu MA, Ulmer JB. Immunogenicity and protective efficacy of a tuberculosis DNA vaccine. Nature Med. 2,893-8,1996.
Kalai M, Montero-Julian FA, Grötzinger J, Wollmer A, Morelle D, Brochier J,Rose-John S, Heinrich PC, Brailly H, Content J. Participation of two Ser-Ser-Phe-Tyr repeats in interleukin-6 (IL-6)-binding sites of the human IL-6 receptor. Eur J Biochem. 238,714-23,1996.
PUBLICATIONS 1997
Denis O, Lozes E, Huygen K. Induction of cytotoxic T-cell responses against culture filtrate antigens in Mycobacterium bovis bacillus Calmette-Guérin-infected mice. Infect Immun. 65,676-84,1997.
Lozes E, Denis O, Drowart A, Jurion F, Palfliet K, Vanonckelen A, De Bruyn J, De Cock M, Van Vooren JP, Huygen K. Cross-reactive immune responses against Mycobacterium bovis BCG in mice infected with non-tuberculous mycobacteria belonging to the MAIS-Group. Scand J Immunol.46,16-26,1997.
O. Denis, E. Lozes, and K. Huygen, Induction of cytotoxic T Cell responses against culture filtrate antigens in Mycobacterium bovis bacillus Calmette Guerin infected mice. Infect.Immun. 65,676 684,1997.
E. Lozes, O. Denis, A. Drowart, F. Jurion, K. Palfliet, A. Vanonckelen, J. Debruyn, M. Decock, J.P. Van Vooren, and K. Huygen, Cross reactive immune responses against Mycobacterium bovis BCG in mice infected with non tuberculous mycobacteria belonging to the MAIS Group. Scand. J. Immunol. 46,16 26,1997.
A.R. Zlotta, A. Drowart, K. Huygen, J. Debruyn, H. Shekarsarai, M. Decock, M. Pirson, F. Jurion, K. Palfliet, O. Denis, F. Mascart, J. Simon, C.C. Schulman, and J.P. Vanvooren, Humoral response against heat shock proteins and other mycobacterial antigens after intravesical treatment with bacille Calmette Guerin (BCG) in patients with superficial bladder cancer. Clin. Exp. Immunol. 109,157 165,1997.
Lefèvre P, Braibant M, de Wit L, Kalai M, Röeper D, Grötzinger J, Delville JP, Peirs P, Ooms J, Huygen K, Content J. Three different putative phosphate transport receptors are encoded by the Mycobacterium tuberculosis genome and are present at the surface of Mycobacterium bovis BCG. J. Bacteriol. 179,2900-6,1997.
Peirs P, De Wit L, Braibant M, Huygen K, Content J. A serine/threonine protein kinase from Mycobacterium tuberculosis. Eur J Biochem. 244,604-12,1997.
Lozes E, Huygen K, Content J, Denis O, Montgomery DL, Yawman AM, Vandenbussche P, Van Vooren JP, Drowart A, Ulmer JB, Liu MA. Immunogenicity and efficacy of a tuberculosis DNA vaccine encoding the components of the secreted antigen 85 complex. Vaccine 15,830-3,1997.
Ulmer, J.B., R.R. Deck, C.M. Dewitt, J.J. Donnelly, A. Friedman, D.L. Montgomery, A.M. Yawman, I.M. Orme, O. Denis, J. Content, K. Huygen, and M.A. Liu. Induction of immunity by DNA vaccination : Application to Influenza and Tuberculosis. Behring Inst. Mitt. 98,79 86,1997.
PUBLICATIONS 1998
Denis O, Tanghe A, Palfliet K, Jurion F, van den Berg TP, Vanonckelen A, Ooms J, Saman E, Ulmer JB, Content J, Huygen K. Vaccination with plasmid DNA encoding mycobacterial antigen 85A stimulates a CD4+ and CD8+ T-cell epitopic repertoire broader than that stimulated by Mycobacterium tuberculosis H37Rv infection. Infect Immun. 66,1527-33,1998.
PUBLICATIONS 1999
A. Tanghe, P. Lefevre, O. Denis, S. Dsouza, M. Braibant, E. Lozes, M. Singh, D. Montgomery, J. Content, and K. Huygen, Immunogenicity and protective efficacy of tuberculosis DNA vaccines encoding putative phosphate transport receptors. J Immunol 162,1113 1119,1999.
Vercammen, M, T. Scorza, A. Elbouhdidi, K. Vanbeeck, Y. Carlier, J.F. Dubremetz, and H. Verschueren, Opsonization of Toxoplasma gondii tachyzoites with nonspecific immunoglobulins promotes their phagocytosis by macrophages and inhibits their proliferation in nonphagocytic cells in tissue culture. Parasite. Immunol. 21,555 563,1999.
PUBLICATIONS 2000
D’Souza, S, O. Denis, T. Scorza, F. Nzabintwali, H. Verschueren, and K. Huygen. CD4(+) T cells contain Mycobacterium tuberculosis infection in the absence of CD8(+) T cells in mice vaccinated with DNA encoding Ag85A. Eur. J. Immunol. 30,2455 2459,2000.
Turner, O.C., A.D. Roberts, A.A. Frank, S.W. Phalen, D.M. Mcmurray, J. Content, O. Denis, S. Dsouza, A. Tanghe, K. Huygen, and I.M. Orme. Lack of protection in mice and necrotizing bronchointerstitial pneumonia with bronchiolitis in guinea pigs immunized with vaccines directed against the hsp60 molecule of Mycobacterium tuberculosis. Infect. Immun. 68,3674 3679,2000.
Tanghe, A, O. Denis, B. Lambrecht, V. Motte, T. Vandenberg, and K. Huygen. Tuberculosis DNA vaccine encoding Ag85A is immunogenic and protective when administered by intramuscular needle injection but not by epidermal gene gun bombardment. Infect. Immun. 68,3854 3860,2000
Y. Fikri, J. Nyabenda, M. Denis and P.-P. Pastoret. Purification and characterization of bovine WC1+ gamma-delta T lymphocytes from peripheral blood. Vet.Res. 31,229-239,2000.
J. Nyabenda, Y. Fikri, O. Denis. Cytokines and granzymes expression in bovine gamma delta T cells are pulsed with Bovine Viral Diarrhoea Virus (BVDV). Abstract. Fifth International Congress of Veterinary Virology. Veterinary Virology in the new Millenium, 2000.
Lefèvre, P, O. Denis, L. Dewit, A. Tanghe, P. Vandenbussche, J. Content and K. Huygen. Cloning of the gene encoding a 22 kilodalton cell surface antigen of Mycobacterium bovis BCG and analysis of its potential for DNA vaccination against tuberculosis. Infect. Immun. 68,1040 1047,2000.
P. Peirs, B. Parmentier, L. De Wit, and J. Content, The Mycobacterium bovis homologous protein of the Mycobacterium tuberculosis serine/threonine protein kinase Mbk (PknD) is truncated. FEMS Microbiol. Lett. 188,135 139,2000.
Vercammen, M, T. Scorza, K. Huygen, J. De Braekeleer, R. Diet, D. Jacobs, E. Saman, and H. Verschueren. DNA vaccination with genes encoding Toxoplasma gondii antigens GRA1, GRA7, and ROP2 induces partially protective immunity against lethal challenge in mice. Infect. Immun. 68,38 45,2000.
PUBLICATIONS 2001
Galamba A, Soetaert K, Wang XM, De Bruyn J, Jacobs P, Content J. Disruption of adhC reveals a large duplication in the Mycobacterium smegmatis mc(2)155 genome. Microbiology 147,3281 94,2001.
Galamba A, Soetaert K, Buyssens P, Monnaie D, Jacobs P, Content J. Molecular and biochemical characterisation of Mycobacterium smegmatis alcohol dehydrogenase C. FEMS Microbiol Lett 196,51 6, 2001.
Y. Fikri, O. Denis, P.-P. Pastoret and J. Nyabenda. Purified bovine WC1+ T lymphocytes are activated by staphylococcal enterotoxins and Toxic Shock Syndrome Toxin-1 superantigens : proliferation response, TCR V gamma profile and cytokines expression. Immunol. lett. 77,87-95,2001.
Tanghe A, Content J, Van Vooren JP, Portaels F, Huygen K. Protective efficacy of a DNA vaccine encoding antigen 85A from Mycobacterium bovis BCG against Buruli ulcer. Infect Immun 69,5403 11,2001.
Tanghe A, D’Souza S,Rosseels V, Denis O, Ottenhoff TH, Dalemans W, Wheeler, C, Huygen K. Improved immunogenicity and protective efficacy of a tuberculosis DNA vaccine encoding Ag85 by protein boosting. Infect Immun 69,3041 7,2001.
PUBLICATIONS 2002
Wang XM, Wilkin JM, Boisteau O, Harmegnies D, Blanc C, Vandenbussche P, Montero Julian FA, Jacques Y, Content J. Engineering and use of 32P labeled human recombinant interleukin 11 for receptor binding studies. Eur J Biochem 269,61 8,2002.
Fikri Y, Pastoret PP, Nyabenda J.Costimulatory molecule requirement for bovine WC1+gammadelta T cells’ proliferative response to bacterial superantigens. Scand J Immunol. 55,373-81,2002.
Walravens K, Marche S, Rosseels V, Wellemans V, Boelaert F, Huygen K, Godfroid J.IFN-gamma diagnostic tests in the context of bovine mycobacterial infections in Belgium. Vet Immunol Immunopathol. 87,401-6,2002.
Denis O, Stroobant V, Colau D, D’Souza S, Huygen K.Culture filtrate specific H-2(b) restricted CD8+ T cells activated in vivo by Mycobacterium tuberculosis or bovis BCG recognize a restricted number of immunodominant peptides. Immunol Lett. 81,115-24,2002.
D’Souza S, Rosseels V, Denis O, Tanghe A, De Smet N, Jurion F, Palfliet K, Castiglioni N, Vanonckelen A, Wheeler C, Huygen K. Improved tuberculosis DNA vaccines by formulation in cationic lipids. Infect Immun.70,3681-8,2002.
PUBLICATIONS 2003
Harmegnies D, Wang XM, Vandenbussche P, Leon A, Vusio P, Grötzinger J, Jacques Y, Goormaghtigh E, Devreese B, Content J. Characterization of a potent human interleukin-11 agonist.Biochem J. 375,23-32,2003.
D’Souza S, Rosseels V, Romano M, Tanghe A, Denis O, Jurion F, Castiglione N, Vanonckelen A, Palfliet K, Huygen K.Mapping of murine Th1 helper T-Cell epitopes of mycolyl transferases Ag85A, Ag85B, and Ag85C from Mycobacterium tuberculosis. Infect Immun. 71,483-93,2003.
Scorza T, D’Souza S, Laloup M, Dewit J, De Braekeleer J, Verschueren H, Vercammen M, Huygen K, Jongert E. A GRA1 DNA vaccine primes cytolytic CD8(+) T cells to control acute Toxoplasma gondii infection.Infect Immun 71,309-16,2003.
Bivas-Benita, M., M. Laloup, S. Versteyhe, J. Dewit, J. De Braekeleer, E. Jongert, and G. Borchard.Generation of Toxoplasma gondii GRA1 protein and DNA vaccine loaded chitosan particles : preparation, characterization, and preliminary in vivo studies. Int J Pharm 266,17-27,2003.
PUBLICATIONS 2004
Romano M, Denis O, D’Souza S, Wang XM, Ottenhoff TH, Brulet JM, Huygen K, Induction of in vivo functional Db-restricted cytolytic T cell activity against a putative phosphate transport receptor of Mycobacterium tuberculosis. J Immunol. 172,6913-21,2004.
Prevot G, Bourreau E, Pascalis H, Pradinaud R, Tanghe A, Huygen K, Launois P. Differential production of systemic and intralesional gamma interferon and interleukin-10 in nodular and ulcerative forms of Buruli disease. Infect Imun.72,958-65,2004.
Allix C, Supply P, Fauville-Dufaux M. Utility of fast mycobacterial interspersed repetitive unit-variable number tandem repeat genotyping in clinical mycobacteriological analysis. Clin Infect Dis. 39,783-9,2004.
Lefèvre P., Peirs P., Braibant M., Fauville-Dufaux M., Vanhoof R., Huygen K., Wang X.-M., Pogell B., Wang Y., Fischer P., Metz P. and Content J. Antimycobacterial activity of synthetic pamamycins. J Antimicrob Chemother. 54,824-7,2004
Doherty TM, Olsen AW, Weischenfeldt J, Huygen K, D’Souza S, Kondratieva TK, Yeremeev VV, Apt AS, Raupach B, Grode L, Kaufmann S, Andersen P. Comparative analysis of different vaccine constructs expressing defined antigens from Mycobacterium tuberculosis. Infect Dis. 190,2146-53,2004
PUBLICATIONS 2005
Peirs P., Lefèvre P., Boarbi S., Wang X-M., Denis O., Braibant M., Pethe K., Locht C., Huygen K. and Content J. Mycobacterium tuberculosis disrupted in its genes encoding the phosphate binding proteins PstS1and PstS2, is deficient in phosphate uptake and demonstrates reduced in vivo virulence. Infect. Immun. 73,1898-1902,2005.
Zozio, T., Allix, C., Gunal, S., Saribas, Z., Alp, A., Durmaz, R., Fauville-Dufaux, M., Rastogi, N. and Sola, C. Genotyping of Mycobacterium tuberculosis clinical isolates in two cities of Turkey : description of a new family of genotypes that is phylogeographically specific for Asia Minor. BMC Microbiol 5,44,2005.
Peirs, P., Lefèvre, P., Vanzebergh, F., Celio, N., Kalai, M. and Content, J. (2005). Effect of phosphate starvation on the expression of the genes encoding the Pst systems from M. tuberculosis and M. bovis BCG. Poster presentation at the International Conference on the Pathogenesis of mycobacterial infections. Stockholm, 30th June 2005.
PUBLICATIONS 2006
Romano, M., Roupie, V., Hamard, M. and Huygen, K. Evaluation of the immunogenicity of pBudCE4.1 plasmids encoding mycolyl-transferase Ag85A and phosphate transport receptor PstS-3 from Mycobacterium tuberculosis. Vaccine 24,4640-4643,2006
Rosseels, V., Marché, S., Roupie, V. Govaerts, M.,Godfroid, J., Walravens, K. and Huygen K. Members of the 30-to 32-kilodalton mycolyl transferase family (Ag85) from culture filtrate of Mycobacterium avium subsp. Paratuberculosis are immunodominant Th1-type antigens recognized early upon infection in mice and cattle. Infect Immun 74,202,2006.
Rosseels, V., Roupie, V., Zinniel, D., Barletta, R.G. and Huygen, K. Development of luminescent M. avium subsp. paratuberculosis for rapid screening of vaccine candidates in mice. Infect.Imm. 74,3684-86,2006.
Rosseels, V., Roupie, V., Zinniel, D., Barletta, R.G. and Huygen, K. Development of luminescent M.avium subsp. paratuberculosis for rapid screening of vaccine candidates in mice. Infect.Imm. 74,3684-86,2006.
Rosseels, V., Marché, S., Roupie, V. Govaerts, M.,Godfroid, J., Walravens, K. and Huygen K. Members of the 30-to 32-kilodalton mycolyl transferase family (Ag85) from culture filtrate of Mycobacterium avium subsp. Paratuberculosis are immunodominant Th1-type antigens recognized early upon infection in mice and cattle. Infect Immun 74,202,2006.
Romano, M., D’Souza, S., Adnet, PY, Laali, R., Jurion, F., Palfliet, K. and Huygen, K. Priming but not boosting with plasmid DNA encoding mycolyl-transferase Ag85A from Mycobacterium tuberculosis increases the survival time of Mycobacterium bovis BCG vaccinated mice against low dose intravenous challenge with M.tuberculosis H37Rv. Vaccine 24,3353-3364,2006.
Romano, M., Roupie, V., Wang, X-M, Denis, O, Adnet, P.-Y, Laali, R. and Huygen, K. Immunogenicity and protective efficacy of tuberculosis DNA vaccines combining mycolyl-tranferase and phosphate transport receptor PstS-3. Immunology 118,321-332,2006.
D’Souza, S., Romano, M., Korf, J., Wang, XM, Adnet, PY and Huygen, K. Partial reconstitution of the CD4+-T-cell compartment in CD4 gene knockout mice restores responses to Tuberculosis DNA Vaccines. Infect Immun 74,2751-2759,2006.
Brudey K, Driscoll JR, Rigouts L, Prodinger WM, Gori A, Al-Hajoj SA, Allix C, Aristimuño L, Arora J, Baumanis V, Binder L, Cafrune P, Cataldi A, Cheong S, Diel R, Ellermeier C, Evans JT, Fauville-Dufaux M, Ferdinand S, Garcia de Viedma D,Garzelli C, Gazzola L, Gomes HM, Guttierez MC, Hawkey PM, van Helden PD, Kadival GV, Kreiswirth BN, Kremer K, Kubin M, Kulkarni SP, Liens B, Lillebaek T, Ho ML,Martin C, Martin C, Mokrousov I, Narvskaïa O, Ngeow YF, Naumann L, Niemann S,Parwati I, Rahim Z, Rasolofo-Razanamparany V, Rasolonavalona T, Rossetti ML,Rüsch-Gerdes S, Sajduda A, Samper S, Shemyakin IG, Singh UB, Somoskovi A, Skuce RA, van Soolingen D, Streicher EM, Suffys PN, Tortoli E, Tracevska T, Vincent V, Victor TC, Warren RM, Yap SF, Zaman K, Portaels F, Rastogi N, Sola C. Mycobacterium tuberculosis complex genetic diversity : mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiol. 6,23,2006.
Allix C, Walravens K, Saegerman C, Godfroid J, Supply P, Fauville-Dufaux M.Evaluation of the epidemiological relevance of variable-number tandem-repeat genotyping of Mycobacterium bovis and comparison of the method with IS6110 restriction fragment length polymorphism analysis and spoligotyping. J Clin Microbiol. 44,1951-62,2006.
Supply P, Allix C, Lesjean S, Cardoso-Oelemann M, Rüsch-Gerdes S, Willery E,Savine E, de Haas P, van Deutekom H, Roring S, Bifani P, Kurepina N, Kreiswirth B, Sola C, Rastogi N, Vatin V, Gutierrez MC, Fauville M, Niemann S, Skuce R, Kremer K, Locht C, van Soolingen D. Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis. J Clin Microbiol. 44,4498-510,2006.
D’Souza, S., Romano, M.,Korf, J., Wang, XM, Adnet, PY and Huygen, K. Partial reconstitution of the CD4+-T-cell compartment in CD4 gene knockout mice restores responses to Tuberculosis DNA Vaccines. Infect Immun 74,2751-2759,2006.
PUBLICATIONS 2007
Tanghe, A., P.Y. Adnet, T. Gartner and K. Huygen. A booster vaccination with M. bovis BCG does not increase the protective effect of the vaccine against experimental Mycobacterium ulcerans infection in mice. Infect. Immun. 75,2642-2644,2007.
Fikri, Y, Nyabenda, J, Content, J. and Huygen, K. 2007. Cloning, sequencing and cell surface expression pattern of bovine immunoreceptor NKG2D and adaptor molecules DAP10 and DAP12. Immunogenetics 95,653-659,2007.
Durmaz R, Zozio T, Gunal S, Allix C, Fauville-Dufaux M, Rastogi N. Population-based molecular epidemiological study of tuberculosis in Malatya, Turkey. J Clin Microbiol. 45,4027-35,2007.
Roupie, V., M. Romano, L. Zhang, H. Korf, M. Y. Lin, K. L. M. C. Franken, T. H. M. Ottenhoff, M. R. Klein, and K. Huygen. Immunogenicity of eight dormancy (DosR) regulon encoded proteins of Mycobacterium tuberculosis in DNA vaccinated mice. Infect. Immun. 75,941-949,2007.
Jongert E., S. De Craeye, J Dewit and K. Huygen. GRA7 provides protective immunity in cocktail DNA vaccines against Toxoplasma gondii. Parasite Immunology 29,445-453,2007.
PUBLICATIONS 2008
Tanghe A, Dangy JP, Pluschke G, Huygen K. Improved Protective Efficacy of a Species-Specific DNA Vaccine Encoding Mycolyl-Transferase Ag85A from Mycobacterium ulcerans by Homologous Protein Boosting. PLoS Negl Trop Dis. 2,199, 2008.
Romano, M., Rindi, L., Korf, H., Bonanni, D., Adnet, P.Y., Jurion, F., Garzelli, C., and Huygen, K. 2008. Immunogenicity and protective efficacy of tuberculosis subunit vaccines expressing PPE44 (Rv2770c). Vaccine 26,6053-6063,2008.
Gartner T., M. Romano, V. Suin, M. Kalai, H. Korf, P. De Baetselier and K. Huygen. Immunogenicity and protective efficacy of a tuberculosis DNA vaccine co-expressing pro-apoptotic caspase-3. Vaccine, 26,1458-1470, 2008.
Jongert E., V. Melkebeek, S. De Craeye, J. Dewit, D. Verhelst and E. Cox. An enhanced GRA1-GRA7 cocktail DNA vaccine primes anti-Toxoplasma immune responses in pigs. Vaccine 26, 1025-1031, 2008
De Craeye S., Francart A., Chabauty J., Van Gucht S., Leroux I. and Jongert E. Toxoplasmosis in Belgian pet cats : recommendations for owners. Flemish Veterinary Journal, 77,325-330, 2008.
De Craeye S., Francart A., Chabauty J., De Vriendt V., Van Gucht S., Leroux I. and Jongert E. Prevalence of Toxoplasma gondii infection in Belgian house cats. Veterinary Parasitology157,128-132, 2008.
Kijlstra A., Meerburg B., Cornelissen J., De Craeye S., Vereijken P. and Jongert E. The role of rodents and shrews in the transmission of Toxoplasma gondii to pigs. Veterinary Parasitology 156,183-90, 2008.
Kijlstra A. and Jongert E. Control of the risk of human toxoplasmosis transmitted by meat. Int.J Parasitol.38,1359-1370, 2008.
Jongert E., Verhelst D., Abady M., Petersen E. and Gargano N. Protective Th1 immune responses against chronic toxoplasmosis induced by a protein-protein vaccine combination but not by its DNA-protein counterpart. Vaccine. 26,5289-5295, 2008.
PUBLICATIONS 2009
Korf H, Vander Beken S, Romano M, Steffensen KR, Stijlemans B, Gustafsson JA,Grooten J, Huygen K. Liver X receptors contribute to the protective immune response against Mycobacterium tuberculosis in mice. J Clin Invest. 119,1626-37,2009.
Jongert E, Roberts CW, Gargano N, Förster-Wald E, Petersen E.Vaccines against Toxoplasma gondii : challenges and opportunities. Mem Inst Oswaldo Cruz. 104,252-66,2009.
Kijlstra A, Jongert E. Toxoplasma-safe meat : close to reality ? Trends Parasitol. 25,18-22,2009.
Rosenberg C, De Craeye S, Jongert E, Gargano N, Beghetto E, Del Porto P, Vorup-Jensen T, Petersen E. Induction of partial protection against infection with Toxoplasma gondii genotype II by DNA vaccination with recombinant chimeric tachyzoite antigens. Vaccine 27, 2489-98, 2009
PUBLICATIONS 2014
Kassem, A., Pays, E. and Vanhamme, L. (2014) ’Transcription is initiated on silent variant surface glycoprotein expression sites despite monoallelic expression in Trypanosoma brucei’, Proc Natl Acad Sci U S A, 111(24), pp. 8943-8.
Pyana Pati, P., Van Reet, N., Mumba Ngoyi, D., Ngay Lukusa, I., Karhemere Bin Shamamba, S. and Büscher, P. (2014) ’Melarsoprol sensitivity profile of Trypanosoma brucei gambiense isolates from cured and relapsed sleeping sickness patients from the Democratic Republic of the Congo’, PLoS Negl Trop Dis, 8(10), pp. e3212.
PUBLICATIONS 2015
Kerkhof, K., Canier, L., Kim, S., Heng, S., Sochantha, T., Sovannaroth, S., Vigan-Womas, I., Coosemans, M., Sluydts, V., Ménard, D. and Durnez, L. (2015) ’Implementation and application of a multiplex assay to detect malaria-specific antibodies : a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas’, Malar J, 14, pp. 338.
Soetaert, K., Rens, C., Wang, X. M., De Bruyn, J., Lanéelle, M. A., Laval, F., Lemassu, A., Daffé, M., Bifani, P., Fontaine, V. and Lefèvre, P. (2015) ’Increased Vancomycin Susceptibility in Mycobacteria : a New Approach To Identify Synergistic Activity against Multidrug-Resistant Mycobacteria’, Antimicrob Agents Chemother, 59(8), pp. 5057-60.
Darcis, G., Kula, A., Bouchat, S., Fujinaga, K., Corazza, F., Ait-Ammar, A., Delacourt, N., Melard, A., Kabeya, K., Vanhulle, C., Van Driessche, B., Gatot, J. S., Cherrier, T., Pianowski, L. F., Gama, L., Schwartz, C., Vila, J., Burny, A., Clumeck, N., Moutschen, M., De Wit, S., Peterlin, B. M., Rouzioux, C., Rohr, O. and Van Lint, C. (2015) ’An In-Depth Comparison of Latency-Reversing Agent Combinations in Various In Vitro and Ex Vivo HIV-1 Latency Models Identified Bryostatin-1+JQ1 and Ingenol-B+JQ1 to Potently Reactivate Viral Gene Expression’, PLoS Pathog, 11(7), pp. e1005063.
PUBLICATIONS 2016
Birhanu, H., Gebrehiwot, T., Goddeeris, B. M., Büscher, P. and Van Reet, N. (2016) ’New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels’, PLoS Negl Trop Dis, 10(4), pp. e0004556.
Caljon, G., Van Reet, N., De Trez, C., Vermeersch, M., Pérez-Morga, D. and Van Den Abbeele, J. (2016) ’The Dermis as a Delivery Site of Trypanosoma brucei for Tsetse Flies’, PLoS Pathog, 12(7), pp. e1005744.
Fikru, R., Matetovici, I., Rogé, S., Merga, B., Goddeeris, B. M., Büscher, P. and Van Reet, N. (2016) ’Ribosomal DNA analysis of tsetse and non-tsetse transmitted Ethiopian Trypanosoma vivax strains in view of improved molecular diagnosis’, Vet Parasitol, 220, pp. 15-22.
Kerkhof, K. (2016) Serological markers to measure recent changes in malaria transmission – a promising tool towards malaria elimination. Ph. D. University of Antwerp, Antwerp, 204 pages
Kerkhof, K., Sluydts, V., Heng, S., Kim, S., Pareyn, M., Willen, L., Canier, L., Sovannaroth, S., Ménard, D., Sochantha, T., Coosemans, M. and Durnez, L. (2016) ’Geographical patterns of malaria transmission based on serological markers for falciparum and vivax malaria in Ratanakiri, Cambodia’, Malar J, 15(1), pp. 510.
Kerkhof, K., Sluydts, V., Willen, L., Kim, S., Canier, L., Heng, S., Tsuboi, T., Sochantha, T., Sovannaroth, S., Ménard, D., Coosemans, M. and Durnez, L. (2016) ’Serological markers to measure recent changes in malaria at population level in Cambodia’, Malar J, 15(1), pp. 529.
Bouchat, S., Delacourt, N., Kula, A., Darcis, G., Van Driessche, B., Corazza, F., Gatot, J. S., Melard, A., Vanhulle, C., Kabeya, K., Pardons, M., Avettand-Fenoel, V., Clumeck, N., De Wit, S., Rohr, O., Rouzioux, C. and Van Lint, C. (2016) ’Sequential treatment with 5-aza-2’-deoxycytidine and deacetylase inhibitors reactivates HIV-1’, EMBO Mol Med, 8(2), pp. 117-38.
Rens, C., Laval, F., Daffé, M., Denis, O., Frita, R., Baulard, A., Wattiez, R., Lefèvre, P. and Fontaine, V. (2016) ’Effects of Lipid-Lowering Drugs on Vancomycin Susceptibility of Mycobacteria’, Antimicrob Agents Chemother, 60(10), pp. 6193-9.
PUBLICATIONS 2017
Darcis, G., Bouchat, S., Kula, A., Van Driessche, B., Delacourt, N., Vanhulle, C., Avettand-Fenoel, V., De Wit, S., Rohr, O., Rouzioux, C. and Van Lint, C. (2017) ’Reactivation capacity by latency-reversing agents ex vivo correlates with the size of the HIV-1 reservoir’, AIDS, 31(2), pp. 181-189.
Fauquenoy, S., Robette, G., Kula, A., Vanhulle, C., Bouchat, S., Delacourt, N., Rodari, A., Marban, C., Schwartz, C., Burny, A., Rohr, O., Van Driessche, B. and Van Lint, C. (2017) ’Repression of Human T-lymphotropic virus type 1 Long Terminal Repeat sense transcription by Sp1 recruitment to novel Sp1 binding sites’, Sci Rep, 7, pp. 43221.
Gama, L., Abreu, C. M., Shirk, E. N., Price, S. L., Li, M., Laird, G. M., Pate, K. A., Wietgrefe, S. W., O’Connor, S. L., Pianowski, L., Haase, A. T., Van Lint, C., Siliciano, R. F., Clements, J. E. and the LRA-SIV Study Group. (2017) ’Reactivation of simian immunodeficiency virus reservoirs in the brain of virally suppressed macaques’, AIDS, 31(1), pp. 5-14.
PUBLICATIONS 2018
Rens, C., Laval, F., Wattiez, R., Lefèvre, P., Dufrasne, F., Daffé, M. and Fontaine, V. (2018) ’I3-Ag85 effect on phthiodiolone dimycocerosate synthesis’, Tuberculosis, 108(Supplement C), pp. 93-95.
Bannister-Tyrrell, M., Srun, S., Sluydts, V. Gryseels, C., Mean, V., Kim, S., Sokny, M., Peeters Grietens, M., Menard, D., Tho, S., Van Bortel, W. and Durnez, L. (2018) ’Importance of household-level risk factors in explaining micro-epidemiology of asymptomatic malaria infections in Ratanakiri Province, Cambodia ’, Sci Rep, 8, pp. 11643.
Mylka, V., Deckers, J., Ratman, D., De Cauwer, L., Thommis, J., De Rycke, R., Impens, F., Libert, C., Tavernier, J., Vanden Berghe, W., Gevaert, K. and De Bosscher, K. (2018) The autophagy receptor SQSTM1/p62 mediates anti-inflammatory actions of the selective NR3C1/glucocorticoid receptor modulator compound A (CpdA) in macrophages, Autophagy, 14(12):2049-2064. DOI : 10.1080/15548627.2018.1495681
PUBLICATIONS 2019
Zeng S, Constant P, Yang D, Baulard A, Lefèvre P, Daffé M, Wattiez R and Fontaine V (2019). Cpn60.1 (GroEL1) Contributes to Mycobacterial Crabtree Effect : Implications for Biofilm Formation. Front. Microbiol. 10:1149. doi : 10.3389/fmicb.2019.01149
Verdikt R, Darcis G, Ait-Ammar A, Van Lint C. (2019) Applications of CRISPR/Cas9 tools in deciphering the mechanisms of HIV-1 persistence. Curr Opin Virol. 38:63-69
Zeng S, Soetaert K, Ravon F, Vandeput M, Bald D, Kauffmann J-M, Mathys V, Wattiez R, Fontaine V. 2019. Isoniazid bactericidal activity involves electron transport chain perturbation. Antimicrob Agents Chemother 63 : e01841-18. https://doi.org/10.1128/AAC.01841-18.
Kula A, Delacourt N, Bouchat S, Darcis G, Avettand-Fenoel V Verdikt R, Corazza F, Necsoi C, Vanhulle C, Bendoumou M, Burny A, De Wit S, Rouzioux C, Rohr O, Van Lint C. (2019) Heterogeneous HIV-1 Reactivation Patterns of Disulfiram and Combined Disulfiram+Romidepsin Treatments. J Acquir Immune Defic Syndr. 80 : 605-613.
PUBLICATIONS 2020
Ait-Ammar A, Kula A, Darcis G, Verdikt R, De Wit S, Gautier V, Mallon PWG, Marcello A, Rohr O, Van Lint C. (2020) Current Status of Latency Reversing Agents Facing the Heterogeneity of HIV-1 Cellular and Tissue Reservoirs. Front Microbiol. 10 : article 3060. doi : 10.3389/fmicb.2019.03060. eCollection 2019
Lange U, Verdikt R, Ait-Ammar A, Van Lint C. (2020) Epigenetic crosstalk in chronic infection with HIV-1. Semin Immunopathol. (2020) doi : 10.1007/s00281-020-00783-3.
Cuypers, B., Dumetz F., Meysman P. , Laukens, K., De Muylder G., Dujardin J-C. and Domagalska M A. (2020) “The Absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samples”. Microorganisms, 8, 1252 ; doi:10.3390/microorganisms8081252
Ouali, R., Valentim de Brito, K.C., Salmon, D. , Bousbata, S. High-Throughput Identification of the Rhodnius prolixus Midgut Proteome Unravels a Sophisticated Hematophagic Machinery. Proteomes 2020, 8, 16
Tomani, J.C.D. , Kagisha, V. , Tchinda, A.T. , Jansen, O. , Ledoux, A. , Vanhamme, L. , Frederich, M. , Muganga, R. , Souopgui, J. The Inhibition of NLRP3 Inflammasome and IL-6 Production by Hibiscus noldeae Baker f. Derived Constituents Provides a Link to Its Anti-Inflammatory Therapeutic Potentials. Molecules 2020, 25, 4693.
PUBLICATIONS 2021
Shey, R.A., Ghogomu, S.M., Shintouo, C.M., Nkemngo, F.N., Nebangwa, D.N., Esoh, K., Yaah, N.E., Manka’aFri, M., Nguve, J.E., Ngwese, R.A., Njume, F.N., Bertha, F.A., Ayong, L., Njemini, R., Vanhamme, L., Souopgui, J. Computational Design and Preliminary Serological Analysis of a Novel Multi-Epitope Vaccine Candidate against Onchocerciasis and Related Filarial Diseases. Pathogens 2021, 10, 99. https://doi.org/10.3390/pathogens10020099
Bougarne N, Mylka V, Ratman D, Beck IM, Thommis J, De Cauwer L, Tavernier J, Staels B, Libert C, De Bosscher K. Mechanisms Underlying the Functional Cooperation Between PPARα and GRα to Attenuate Inflammatory Responses. Front Immunol. 2019 Aug 9 ;10:1769. doi : 10.3389/fimmu.2019.01769. PMID : 31447832 ; PMCID : PMC6695567.
Tomani, J.C.D. ; Bonnet, O. ; Nyirimigabo, A. ; Descham s,W. ; Tchinda, A.T. ; Jansen, O. ; Ledoux, A. ; Mukazayire, M.J. ; Vanhamme, L. ; Frédérich, M. ; Muganga, R. and Souopgui, J. In Vitro Antiplasmodial and Cytotoxic Activities of Compounds from the Roots of Eriosema montanum Baker f. (Fabaceae). Molecules 2021, 26, 2795. https://doi.org/10.3390/molecules26092795
Ouali R, Vieira LR, Salmon D, Bousbata S. Early Post-Prandial Regulation of Protein Expression in the Midgut of Chagas Disease Vector Rhodnius prolixus Highlights New Potential Targets for Vector Control Strategy. Microorganisms. 2021 Apr 11 ;9(4):804. doi : 10.3390/microorganisms9040804.
PUBLICATIONS 2022
Yengo, B.N.; Shintouo, C.M.; Hotterbeekx, A.; Yaah, N.E.; Shey, R.A.; Quanico, J.; Baggerman, G.; Ayong, L.; Vanhamme, L.; Njemini, R.; et al. Immunoinformatics Design and Assessment of a Multiepitope Antigen (OvMCBL02) for Onchocerciasis Diagnosis and Monitoring. Diagnostics 2022, 12, 1440. https://doi.org/10.3390/diagnostics12061440
PUBLICATIONS 2023
Shintouo CM, Shey RA, Yengo BN, Yaah NE, Teh RN, Ngwese RA, Ayong L, Vanhamme L, Souopgui J, Ghogomu SM, Njemini R. Effects of the suspension of mass drug administration during the COVID-19 pandemic on onchocerciasis prevalence in the Bandjoun and Massangam health districts, West Region of Cameroon. Acta Trop. 2023 Aug 5:106999.http://doi: 10.1016/j.actatropica.2023.106999. Epub ahead of print. PMID: 37549841.
Ouali R, Bousbata S. Unveiling the Peptidase Network Orchestrating Hemoglobin Catabolism in Rhodnius prolixus. Mol Cell Proteomics. 2024 Jun;23(6):100775. doi: 10.1016/j.mcpro.2024.100775. Epub 2024 Apr 23. PMID: 38663568; PMCID: